THREE YEARS OPEN POST-DOCTORAL POSITIONS

 

A 3 years post-doctoral position funded by the Labex IBEID is available in the team of Ali AMARA. Start date is from June 2016.

This project aims to decipher the molecular mechanisms regulating flavivirus entry program using Dengue and Zika viruses as a model. Our objective is to use a combination of gain and loss of function screening and proteomics techniques in order to fish new flavivirus entry receptors and to study their function in virus binding and internalization. This project will be performed in collaboration with Olivier Schwartz' Lab (Virus and Immunity Unit, Pasteur Institute).

For this position, a doctoral degree, experience in cellular and molecular biology, virology, CRISPR Cas9 technology, and proficiency in English, are required. Interested candidates should send a motivation letter, a CV, a list of publications and 2 reference letters to A. Amara (Cette adresse e-mail est protégée contre les robots spammeurs. Vous devez activer le JavaScript pour la visualiser.).

Ali Amara

Selected Publications

Fernandez-Garcia, MD, Meertens L, Chazal M, Hafirassou ML, Dejarnac O, Zamborlini A, Despres P, Sauvonnet N, Arenzana-Seisdedos F, Jouvenet N, Amara A. (2016) Vaccine and wild-type strains of yellow fever virus engage distinct entry mechanisms and differentially stimulate antiviral immune responses. mBio, Volume 7 Issue 1 e01956-15.

Amara A, Mercer J (2015).Viral apoptotic mimicry. Nat Rev Microbiol.13(8):461-9.

Hamel R, Dejarnac O, Wichit S, Ekchariyawat P, Neyret A, Luplertlop N, Perera-Lecoin M,Surasombatpattana P,Talignani L, Thomas F, Cao-Lormeau VM, Choumet V,Briant L, Desprès P, Amara A, Yssel H, Missé D. (2015) Biology of Zika virus, J. Virol., Sep 1;89(17):8880-96.

Carnec X, Meertens L, Dejarnac O, Perera-Lecoin M, Hafirassou ML, Kitaura J, Ramdasi R, Schwartz O, Amara A. (2015) The Phosphatidylserine and Phosphatidylethanolamine Receptor CD300a Binds Dengue Virus and Enhances Infection. J Virol. Oct 14;90(1):92-102.

Meertens L, Carnec X, Perera Lecoin M. , Ramdasi R, Guivel-Benhassine F, Lew E, Lemke G, Schwartz O and Amara A. (2012) TIM and TAM mediate Dengue virus infection. Cell Host & Microbe, 12, 4, 544-557.

FRM FUNDED COMPUTATIONAL ENGINEER- PROCESSING AND 3D IMAGE ANALYSES IN FLUORESCENCE MICROSCOPY- ST LOUIS HOSPITAL, UMR 7212, PARIS, FRANCE

Scientific environment

Our team “Chromosome Biology and Dynamics” belongs to the UMR7212, Pathology and Molecular Virology, composed of 6 teams, internationally recognized, implicated in many aspects of Cancer, Virology and Cellular Biology. The UMR7212 is backed to the University Institute of Haematology and his technological platform in the St Louis Hospital. Our team “Chromosome Biology and Dynamics” has a long-standing experience in the functional organization of chromosomes in the nuclear space.  We are currently focusing on the temporal features of chromosomes upon cell differentiation and DNA damages. Our interdisciplinary team has expertise in both unicellular eukaryotic yeasts and embryonic stem cells allowing a unique cross talk between organisms. To address our questions, we intensively use optical fluorescence microscopy to visualize in real time different processes in our models. We have recently acquired an advanced fluorescent microscopy station allowing 3D Imaging + t in single cells.  Biological observations are routinely confronted with physics polymer models.

We are looking for a highly motivated processing and image analysis engineer to analyse the large multidimensional microscopy data sets we are generating to investigate multiple aspects of nuclear morphology, gene position and motion. The engineer should show autonomy and creativity to conduct his/her analyses.

Missions
• Design and implement tools for image analysis and processing using ImageJ /Fiji / Icy and Matlab.
• Develop novel strategies for analysis of multidimensional microscopy datasets
• Provide and Perform adequate statistical analyses
• Set-up advanced workflow. e.g. image segmentation, quantify intracellular protein
distribution, pattern recognition, tracking of dynamic particle …
• Make tutorials and train scientists in the use of developed tools.

Skills
• University degree (Master, PhD), engineering school or equivalent degree with a strong specialization in image processing and analysis.
• Proven experience in the development of tools to process and analyse
multidimensional fluorescence microscopy datasets using ImageJ / Fiji / ICY (macros and java plugin) and Matlab.
• Good knowledge of segmentation algorithms, object tracking, pattern recognition.
• Ability to work in team and have interest and understanding in biology.
• Ability to manage multiple projects simultaneously. A high degree of autonomy is required.
• Able to communicate in English.

Please send your CV, cover letter and contact information for two references to Cette adresse e-mail est protégée contre les robots spammeurs. Vous devez activer le JavaScript pour la visualiser.. Contract for 12 months (renewable 12 months). Salary depending on experience and qualifications.
Applications will be reviewed when they are received.

 

 

 

 

 

 

 

TWO OPEN POST-DOCTORAL POSITIONS

 

Plasticity of the Nuclear Pore Complex and gene expression

A 2 years postdoctoral position funded by ANR is available in the team of Catherine Dargemont. Start date is from May 2016.

This project focuses on the role of post-translational modifications of the NPC on the gene expression program in yeast. A specific expertise in cellular biology and advanced microscopy is required in order to tackle this project at a single cell level.

 

The Nuclear Pore Complex in the DNA damage response

A 18-months postdoctoral position funded by INCA is available in the team of Catherine Dargemont. Start date is from May 2016.

This project aims to provide insight into the understanding of how the different components of the nuclear pore complex are orchestrated to preserve genomic integrity and coordinate DNA repair with chromosome architecture. We approach these fundamental cellular questions in both yeast and human cells through a consortium made by three groups leaded by C. Dargemont, E. Soutoglou, V. Géli with unique, complementary and synergistic areas of expertise.

For both positions, a doctoral degree, experience in cellular and molecular biology, and proficiency in English, are required. Interested candidates should send a motivation letter, a CV,

a list of publications and three reference letters to C. Dargemont (Cette adresse e-mail est protégée contre les robots spammeurs. Vous devez activer le JavaScript pour la visualiser.).

 

Catherine Dargemont

 

Selected Recent Pubications

Niño et al. (2016) Posttranslational marks control architectural and functional plasticity of the nuclear pore complex basket. (2016) J. Cell Biol. 212:167-180. 


Guet et al. (2015) Combining Spinach-tagged RNA and gene localization to image gene expression in live yeast. Nature Commun. 6:8882, doi: 10.1038/ncomms9882. 


Bonizec et al. (2014) The Ubiquitin-Selective Chaperone Cdc48/p97 Associates with Ubx3 to Modulate Monoubiquitylation of Histone H2B. Nucleic Acids Res. 42(17):10975-86. 


Ossareh-Nazari et al. (2014) Ubiquitylation by the Ltn1 E3 ligase protects 60S ribosomes from nutrient starvation-induced selective autophagy. J. Cell Biol. 204(6): 909-917. 


Margaritis et al. (2012) Dimethymethyletion of H3K4 by Set1 represses transcription of a subset of coding genes by promoting 3’-end antisense RNA production. PLoS Genetics. 8(9): e1002952. 


Vitaliano-Prunier et al. (2012) Mol Cell. 1: 132-139. H2B ubiquitylation controls the formation of export-competent mRNP. 


Hayakawa, A. et al. (2012) Ubiquitylation of the nuclear pore complex controls nuclear migration during mitosis in S. cerevisiae. J. Cell Biol. 1:19-27. 


 

  1. INSERM U944 - CNRS UMR 7212

    Hôpital Saint Louis. 1, ave. Claude Vellefaux - 75475 Paris Cedex 10 - FRANCE

  2. Tél : 33.1.53724046 Fax : 33.1.53724027